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KMID : 0381120120340030299
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Genes and Genomics
2012 Volume.34 No. 3 p.299 ~ p.304
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DNA methylation of stem cell surface markers in colon cancers
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Yi Joo-Mi
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Abstract
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DNA methylation is considered an important mechanism for inactivation of tumor suppressor genes in the cancer genome. Recent advances in genome-wide analysis technology have identified a number of new genes that are inactivated in cancer and regulated by DNA hypermethylation. This study investigates transcriptional silencing of the CD44, CD34, and EpCAM genes, which are well known as cancer stem cell surface markers in many types of cancer. We examined promoter DNA methylation patterns for CpG island for CD44, CD34, and EpCAM genes in eleven colon cancer cell lines by methylation specific PCR (MSP). LoVo and Colo205 cells showed robust promoter DNA hypermethylation, which strongly correlated with gene re-expression after 5-Aza-2′-deoxycytidine (DAC) treatment. We also confirmed promoter hypermethylation of CD44, CD34, and EpCAM genes by bisulfite sequencing in LoVo cells compared with DNMT1 (?/?) DNMT3B (?/?) double knockout (DKO) HCT116 cells and normal colon tissue. This data suggest that the promoter DNA hypermethylation seen in colon cancer is cancer-specific. Taken together, transcriptional silencing of CD44, CD34, and EpCAM gene is caused by promoter DNA hypermethylation of their CpG islands in colon cancer cell lines. Our data might contribute to understanding the biological roles of inactivating cancer stem cell markers in colon cancers.
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KEYWORD
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DNA methylation, Stem cell surface markers, Colon cancer, Gene expression, Transcriptional silencing
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